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KJM5240 - Mass spectrometry

Course content

The student will acquire a thorough familiarity with the physical and chemical basis of mass spectrometry, and with analytic use of mass spectrometry in organic chemistry and biochemistry.

Learning outcomes

On succesful completion of this course:

• you will have a deep knowledge about he principles behind the most common mass analysators: magnetic (double focusing), quadrupol, ion trap, ion cyclotron resonance (ICR and FT-ICR), time-of-flight analysator (TOF), and the combinations GC/MS and LC/MS.
• you will know the principles of the main ionisation methods: electron ionisation (EI), chemical ionisation (CI), field ionisation (FI), photo ionisation (PI), fast atom bombardement (FAB), secundary ion mass spectrometry (SIMS), field desorption (FD), plasma desorption (PD), laser desorption (LD and MALDI), and electrospray ionisation (ESI).
• you will have detailed knowledge about the physico-chemical principles of mass spectrometry: kinetics and energy in monomolecular reactions, energy distribution, reaction mechanisms, ion structure, metastable ions, collision activation, tandem mass spectrometry (MS/MS), isotope effects – also ion/molecule reactions, proton affinity, acidity and basicity in the gas phase.
• you will be able to interpret mass spectra and know the principles of fragmentation. Examples are selected both from simple organic compounds and from more complicated biological compounds (peptides, proteins).

Other topics are analysis of mixtures, quantitative analysis, data processing, and library searching.

Admission

Students at UiO must apply for courses in StudentWeb.

International applicants, if you are not already enrolled as a student at UiO, please see our information about admission requirements and procedures for international applicants.

Prerequisites

Recommended prior knowledge

KJM1110 - Organic chemistry I or equivalent courses.

Overlap

10 credits against KJM9240 - Mass spectrometry.

Teaching

The course consists of 30 hours of lectures and 15 hours of seminars.

The first lecture is mandatory. If you are unable to attend, the Expedition Office has to be informed in advance (phone 22 85 54 46, or e-mail ekspedisjonen@kjemi.uio.no), or else you will lose your place in the course.

Exam information

Oral examination.

Information about examination rules

Assessment and grading

Course grades are awarded on a descending scale using alphabetic grades from A to E for passes and F for fail. Read more about the grading system .

Explanations and appeals

A student may appeal against an awarded grade and/or file a formal complaint about exam procedures.

Possibility of make-up exams and re-takes

This course offers new examination in the beginning of the subsequent term for candidates who withdraw during an ordinary examination or fail an ordinary examination. For general information about new examination, see:

http://www.mn.uio.no/studier/admin/eksamen/utsatt-og-ny-eksamen/index.html

http://www.matnat.uio.no/english/studies/examination/repeat.html

Exam options for students with special needs

Students may apply for access to alternative exam resources or exam forms on the basis of chronic illness and/or special needs that create a marked disadvantage to other students in the exam situation. Mothers who are breastfeeding may apply for extra time to complete the exam.

Other information

The teaching may be cancelled if less than 5 students register for the course.

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