• Studies
• Courses
• • MBV4250
    • Tidligere eksamensoppgaver

MBV4250 - Basic immunology and immunological techniques

Course content

The course is taught over a period of 3 weeks.
During the first week a series of lectures give an introduction to the immune system. Structure, function and regulation of the different molecules of the immune system are described. Furthermore, B cells, T cells, NK cells as well as antigen presenting cells will be presented. Finally, lymphoid organs, cell collaboration and immunological tolerance are discussed.
The second and third week are dedicated to practical laboratory work, demonstrations and seminars. The students will get to clone antibody and T cell receptor V genes by way of PCR and analyse the gene sequences. The genes will be expressed in E.coli and the molecules produced analysed by ELISA with different clonotypic and conformation specific monoclonal antibodies. Furthermore, libraries of V genes will be expressed and displayed on phage and a specificity selected by panning on antigen. During the course other ELISAs will be designed, as well as Western blot, FLOW cytometry and Biacore analysis which will be done partly as demonstrations. A number of techniques which do are not done by the students, such as immunisation and antibody production, production of monoclonal antibodies and recombinant antibodies, cellular methods and cell culture.

Learning outcomes

The course is designed to give an understanding of the basic principles of modern immunology and an introduction to methods used in immunological research.

Admission

Students at UiO must apply for courses in StudentWeb.

International applicants, if you are not already enrolled as a student at UiO, please see our information about admission requirements and procedures for international applicants.

Prerequisites

Recommended prior knowledge

Bachelor degree in molecular biology/biochemistry and/or admission to Master degree in molecular biology/biochemistry/physiology or equivalent background.
MBV3010 - Advanced cell biology, MBV4010 - Methods in molecular biology and biochemistry I, MBV4020 - Methods in molecular biology and biochemistry II or corresponding knowledge

Teaching

The course format will be lectures, colloquia, demonstrations and laboratory exercises.

Exam information

Three hours written exam apr. two weeks after the end of the course.

Former exams

more information on examination from the MN Faculty

Exam resources

No special exam resources are allowed.

Assessment and grading

Course grades are awarded on a descending scale using alphabetic grades from A to E for passes and F for fail. Read more about the grading system .

Explanations and appeals

Students can request an explanation of their grades, and can also appeal against their grades or make a complaint about formal examination errors. Read more about explanations and appeals

Possibility of make-up exams and re-takes

New exam will be offered for students who fails or withdraw from the ordinary exam. Date for new exam will be in agreement with students.

You can usually resit an exam, but the conditions depend on whether you had a valid reason for absence from the regular exam. Read more about resitting an exam .

Withdrawing from exams and limits on re-takes

A student can sit for this exam up to 3 times. If a student wishes to withdraw from the exam, s/he must do this in StudentWeb at least two weeks prior to the first day of the exam. Failure to do so will be counted as one of the three opportunities to sit for the exam.

Exam options for students with special needs

Students may apply for access to alternative exam resources or exam forms on the basis of chronic illness and/or special needs that create a marked disadvantage to other students in the exam situation. Mothers who are breastfeeding may apply for extra time to complete the exam.

Contact us