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MBV9520 - BIOSTRUCT - Advanced Biomolecular NMR

Course content

The course will give a practical introduction to NMR spectroscopy and how it is used to obtain structures of small and large proteins.

Structure information is essential for understanding the function of a protein. For proteins that are difficult or impossible to crystallize NMR spectroscopy is the method available for 3D structure determination.

In NMR spectroscopy resonance frequency, intensity and correlation is measured. These spectroscopic qualities contain structural information. It is this structural information that is used in structure calculations that in the end give the NMR structures. The resolution obtainable from unlabeled NMR samples is not good enough for proteins for the information to be obtained. To obtain NMR structures of proteins it is practice to label the sample with 15N and 13C, as a result there is a whole array of NMR experiments that can be used to obtain structure information. These experiments use direct J-couplings trough bond to connect spins on neighboring nuclei. Experiments on labeled samples can be used to determine the structure of large proteins.

Learning outcomes

Students will learn how to obtain NMR data of labeled proteins on BRUKER NMR instruments. The theory and simple setup of the NMR pulse sequences will be thought. The student should be able to analyze pulse sequences and fix NMR experiments that do not give the wanted outcome. The student will learn to analyze and assign labeled proteins using CARA and automated procedures. Furthermore, the students will learn how to calculate NMR structures using CYANA.

Admission

To participate in this course you must have an admission to a Ph.D.-programme at a Norvegian University or University College.

If you do not have an admission to the Ph.D.-Programme in Science at The Faculty of Mathematics and Natural Sciences at UiO, you have to apply as a visiting PhD student.

For more information, please contact the department.

With free capacity the course is also open for PhD-students from other countries. Please contact Dr.Scient Per Eugen Kristiansen for more information about the course or Torill Rørtveit for questions about admission.
Only students admitted to the course may take part in instruction and tuition and sit for the examination.

Prerequisites

Recommended prior knowledge

The students must have followed the MBV9510 course or have NMR knowledge at similar level.

Overlap

No overlap with other courses.

Teaching

The course comprises for each student of 20 lecture hours and 33 hours of lab-exercises. The course is taught intensively for one 9 consecutive days every second year. First time taught March 2011.
A selected set of home work is described that the student should finish and bring to the final exam. Estimated time for this is 1 week of work.
All teaching sessions are obligatory and constitute the curriculum together with hand-outs and pre-defined articles

Exam information

Approved participation and laboratory journals. Final oral examination counting 100% after course ending

Exam resources

No special exam resources are allowed.

Assessment and grading

Students are awarded either a passing or a failing grade. Read more about the grading system .

Explanations and appeals

Students can request an explanation of their grades, and can also appeal against their grades or make a complaint about formal examination errors. Read more about explanations and appeals

Possibility of make-up exams and re-takes

You can usually resit an exam, but the conditions depend on whether you had a valid reason for absence from the regular exam. Read more about resitting an exam .

Exam options for students with special needs

Students may apply for access to alternative exam resources or exam forms on the basis of chronic illness and/or special needs that create a marked disadvantage to other students in the exam situation. Mothers who are breastfeeding may apply for extra time to complete the exam.

Other information

The course is part of the BioStruct PhD-school (National Graduate School in Structural Biology).

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