MEDFL5135 - Biomarkers for clinical research
The purpose of the course is to introduce the use of biomarkers in clinical research today and tomorrow, as well as presenting the principles for quantification in selected analyses. Such understanding is fundamental for the selection of appropriate choice of analysis and interpretation of data obtained.
Biomarkers can be plasma molecules, cellular molecules, morphologies, enzymatic activities and organelle integrity. Biomarker quantifications will be exemplified as applied in specific techniques, and illustrated in distinct disciplines. Analyses that are introduced will differ in automatization: from large-scale platform instruments (medical biochemistry), to manpower-intensive techniques. The principles behind the following assessment methods for biomarker quantifications will be presented: antibody-based detection methods (Elisa, Western), nucleic acid-based techniques (PCR, RFLP, FISH), mass spectrometry analyses (MALDI TOF), suspension techniques (flow cytometry), imaging techniques (laser capture microscopy).
Included is also a brief introduction of the formal requirements for initiating a clinical research project.
The candidate will learn how to initiate a clinical research study, learn how platform analyses of selected biomarkers are dependent on proper choice of method and proper sample preparation, learn underlying principles in quantification of biomarkers in central techniques designed on subcellular fractions, learn to critically judge the robustness of the data from the choice of method.
Knowledge and skills
This course will give you knowledge about:
- Overview of formal requirements for performing research on human material, including Norwegian Health Research Act
- Biomarkers heterogeneity: what to measure depends on the stability of the sample, resolution of method and preparation of the sample.
- Understanding the underlying principles of antibody-based analyses
- Understanding the underlying principles of nucleic acid-based techniques
- Understanding the underlying principles of functional assays and correlating pitfalls.
- Understanding the underlying principles of mass-spectrometry analyses and
- Understand the principles, limitations and pitfalls associated with platform analyses
- Critical selection of method.
The course is restricted to students at the Medical Student Research Programme at the Faculty of Medicine and the Faculty of Dentistry, UiO.
Students apply in StudentWeb
The courses MEDFL5135 and MF9135 have common admission.
5 credits overlap with MF9135 - Biomarkers in clinical research
The course will run for 5 full days and mainly based on lectures supplemented with e-learning course and group work.
Teaching is mainly by lectures. There will be group work based on critical reviewing of published literature methods, as well as e-learning (Norwegian Health Research Act).
The compulsory literature will be provided after registration, and include a mixture of hand-outs, published literature and web-based resources.
You have to participate in at least 80 % of the teaching to be allowed to take the exam. Attendance will be registered.
A take-home exam will be given at the end of the course. There will be 1-2 days of preparatory.
The exam paper is in English, but it is optional for the candidate to answer the exam in Norwegian or English
Grades are awarded on a pass/fail scale. Read more about the grading system.
Explanations and appeals
Resit an examination
Withdrawal from an examination
It is possible to take the exam up to 3 times. If you withdraw from the exam after the deadline or during the exam, this will be counted as an examination attempt.
Special examination arrangements
Application form, deadline and requirements for special examination arrangements.
The course is subject to continuous evaluation. At regular intervals we also ask students to participate in a more comprehensive evaluation.