MF9225 - A molecular approach to genetic and epigenetic gene regulation, from basic research to the clinic
In depth understanding of molecular methods used for addressing genetic and epigenetic phenomena in basic research and in the clinic.
The understanding of the bases for genetic and epigenetic inheritance has been improved tremendously over the last decades. While Gregor Mendels results completely revolutionized the basic understanding of genetic inheritance with his discoveries in garden pees more than 150 years ago, the understanding of inheritance has recently been challenged by the many dynamic chemical modifications on histones, DNA and RNA that regulate gene expression. Such modifications are able to switch on and off genes without changing the coding sequence of DNA. The very fast switches of individual or group of genes being expressed following fertilization and during development can now be studied in model organisms. What is more, these rapid switches in gene regulation that is required for cellular homeostasis and normal development can also be incorrectly regulated and lead to dysregulation of the cell cycle and cancer.
This course provides an introduction to the various phenomena of genetic and epigenetic inheritance, an updated presentation of novel aspect of epigenetic regulation and examples of epigenetic analysis, including diagnostics and chemotherapeutic agents that have entered the clinic.
Studying genetic and epigenetic regulation in the laboratory is challenging and requires state-of-the-art methodological insight. There will be a particular focus on novel molecular methods used to address these phenomena that includes; analysis of modified bases in DNA and RNA and histone modifications, base-specific DNA sequencing, CHiP sequencing, CRISPR technology, mutant organisms and clinical samples.
- Basic understanding of genetic inheritance
Overview of epigenetic inheritance “coded by” proteins, DNA and RNA
Insight in molecular methods used to address the two above mentioned regulatory mechanisms.
Novel insight in recent publications on:
DNA modifications on cytosine and adenine
RNA mediated epigenetic phenomena
Reversible modifications on protein, DNA and RNA
Epigenetic regulation following fertilization and development
Epigenetic regulation in disease
- Diagnostics and chemotherapeutic epigenetic
PhD candidates at UiO will get first priority to the course. Maximum number of participants is 30.
PhD candidates admitted to a PhD programme at UiO apply in StudentWeb
Applicants who are not admitted to a PhD programme at UiO must apply for a right to study in SøknadsWeb before they can apply for PhD courses in medicine and health sciences.
Formal prerequisite knowledge
The candidates need basic knowledge in genetics and molecular biology.
The teaching will start with overview lectures of genetics and epigenetics and continue with more specific introduction to novel principles of epigenetics. During the last 2/3 days of the course, students will present recent findings of epigenetics with a focus on molecular methods.
You have to participate in at least 80 % of the teaching to be allowed to take the exam. Attendance will be registered.
A home exam will be given at the end of the course.
Use of sources and citation
Language of examination
The examination text is given in English, and you submit your response in English.
Grades are awarded on a pass/fail scale. Read more about the grading system.
Explanations and appeals
Resit an examination
Withdrawal from an examination
It is possible to take the exam up to 3 times. If you withdraw from the exam after the deadline or during the exam, this will be counted as an examination attempt.
The course is subject to continuous evaluation. At regular intervals we also ask students to participate in a more comprehensive evaluation.