3DG_Phys - Physical aspects to 3D modeling of the genome
Genetic information is encoded in DNA, a 2-m long polymer folded into a micrometer-size nucleus in eukaryotic (human) cells. To achieve the necessary compaction, DNA packaging mediated at many levels by molecules which bend, wrap and loop DNA. Folding leads to an array of three-dimensional (3D) conformations of the genome in a population of cells. Proper positioning of interactions between chromosomes and between chromosomes and the nuclear envelope is crucial for proper regulation of gene expression. Next-generation sequencing technologies enable mapping these interactions along the genome; however there is little information on how these interactions are mechanistically controlled. Models of 3D chromosome conformation have been proposed, but these have insufficient “physical reality”. Here, I propose to study the mechanics driving the 3D arrangement of the genome. I intend to add physical parameters into statistical models of the 3D genome elaborated in the host lab, to better simulate 3D folding. I aim to probe the impact of these parameters on the system and extract the most significant ranging effects for the 3D organization of chromosomes. The project proposes a truly original way to investigate the physics of chromatin because the host laboratory and I will constitute a team with unique multidisciplinary complementary expertise.
Prosjektet har ingen godkjenninger