Generation of adipose tissue from induced pluripotent stem cells for "organ on a chip" applications

The overall aim of this study is to differentiate human iPSCs towards an adipogenic fate, white, brown or beige adipocytes that can be implemented on an “organ on a chip” platform.

The subgoals of this study are listed below

  • Differentiation of human induced pluripotent stem cells to an adipogenic fate, white, brown or beige adipocytes
  • Generation of adipose spheroids
  • Implementation of the spheroids on the chip platform to study core energy metabolism
  • Implement the adipose spheroids on the chip platform together with pancreas and liver organoids
  • Generation of reporter cell lines to oversee signaling of the Wnt and hippo pathways

The chip platform will reproduce core energy metabolism in metabolism regulating organs, namely the micro adipose tissue and will enable the acquisition of real time data upon exposure to therapeutic tools and drugs. Among these drugs are Metformin, antidiabetic drugs and drugs that affect the Wnt signaling pathway, such as tankyrase inhibitors. Especially the transdifferentiation of white adipocytes to beige adipocytes will be investigated.

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Prosjektbeskrivelse med vedlegg

Disse dokumentene er kun synlige for prosjektleder, enhetens leder og forskningsadministrasjon.

TSD

  • Ja

Biobank

  • Lagret: Department of Cellular Therapy, Radiumhospitalet, Oslo
  • Biobanknummer: biobank for somatiske stemceller REK 2013/924
  • Ansvarlig: Hanne Scholz (OUS/UiO)

Godkjenninger

Intern fremleggelse - Ja1 fil

Disse dokumentene er kun synlige for prosjektleder, enhetens leder og forskningsadministrasjon.

REK - Ja 2 filer

Disse dokumentene er kun synlige for prosjektleder, enhetens leder og forskningsadministrasjon.

Tidspunkt for anonymisering og sletting av dataene

  • Sletting: oktober 2037

Prosjektleder / prosjektansvarlig ved UiO

Stefan Krauss

Ansvarlig enhet

SFF - Hybrid Technology Hub

Forskere

Prosjekttype

  • Ph.d.-prosjekt
  • Forskerprosjekt

Helsefaglig forskning

  • Ja

Personopplysninger

  • Ikke besvart

Tidsperiode

  • Start: oktober 2018
  • Slutt: desember 2032