Genetic and functional analysis of bone marrow CD34+ and CD34+-derived blood lineage cells
The lifelong formation of blood cells requires continuous self-renewal of the hematopoietic stem cell (HSC) pool, and functional differentiation programs. This is guaranteed by asymmetric cell division of HSC that give rise to HSC and CD34+ progenitor cells with limited renewing capacity. The progenitors then produce daughter cells that are restricted to specific lineages and form terminally differentiated cells of myeloid and lymphoid lineages. Here, we will isolate bone marrow CD34+ cells, and differentiate those into different blood lineage cells (erythroid precursor cells, monocytes, granulocytes, T cells and NK cells). We will use CD34+ and in vitro differentiated lineage cells to study chromatin dynamics underlying blood cell differentiation to identify the transcriptional and epigenetic network required for hematopoietic differentiation.
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