MBV9220 – Protein Crystal Spectroscopy

Schedule, syllabus and examination date

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Course content

The course gives and introduction to protein crystal spectroscopy, which is used in combination with protein crystallography to study the structure and function of redox- and metalloproteins. For these types of proteins the structures determined by protein crystallography do not to fully describe the state of these proteins. The essential information that is missing is the oxidation state, spin-state, protonation and in some cases the coordination. This information is provided by the spectroscopic and theoretical methods taught in this course. The course is part of the PhD School in Structural Biology (BioStruct) .

Learning outcome

After completing this course we expect your competence to include the following:

  • Know the theory for UV-vis and Raman single-crystal protein spectroscopy, and how to use these methods to study redox- and metalloproteins.
  • Have knowledge about the theory for electron paramagnetic resonance (EPR) and theoretical methods (density function theory, QM/MM, quantum refinement) for the study of proteins, and how to use these methods.
  • Know how X-rays used to solve the crystal structure of proteins can lead to radiation damage of redox- and metallorproteins, and how to use spectroscopy to monitor these effects.
  • Know how to combine these methods for studying protein structure, and how to use them in-situ at the synchrotrons.
  • Be able to use the above methods in practice.

Admission

PhD candidates from the University of Oslo should apply for classes and register for examinations through Studentweb.

If a course has limited intake capacity, priority will be given to PhD candidates who follow an individual education plan where this particular course is included. Some national researchers’ schools may have specific rules for ranking applicants for courses with limited intake capacity.

PhD candidates who have been admitted to another higher education institution must apply for a position as a visiting student within a given deadline.

Only students admitted to the course may take part in instruction and tuition and sit for the examination.

Prerequisites

Recommended previous knowledge

Basic knowledge of protein structure and protein structure determination.

Overlapping courses

No overlap with other courses.

Teaching

The course comprises for each student of 21 lecture hours and 16 hours of lab-exercises. The course is taught intensively for one week in the beginning of the autumn term every second year.

A selected set of articles should be studied before course start. Estimated time for this activity: one week.

All teaching sessions are obligatory and constitute the curriculum together with hand-outs and pre-defined articles

Examination

Approved participation and laboratory journals. Internet examination through Canvas after course ending.

Examination support material

No examination support material is allowed.

Grading scale

Grades are awarded on a pass/fail scale. Read more about the grading system.

Explanations and appeals

Resit an examination

This course offers both postponed and resit of examination. Read more:

Special examination arrangements

Application form, deadline and requirements for special examination arrangements.

Other

The course was earlier part of the BioStruct PhD-school (National Graduate School in Structural Biology) with the course code MBV9220BIOSTRUCT

Facts about this course

Credits

5

Level

PhD

Teaching

Every other autumn starting 2010

Examination

Every other autumn starting 2010

Teaching language

English