Molecular mechanisms of cytokinetic abscission in vivo
Cytokinesis is the final step of cell division and concludes with abscission, which involves cleavage of the thin intercellular bridge connecting the two daughter cells. Fidelity in cell division and cytokinesis is essential to ensure proper partitioning of the genetic material between the two daughter cells.
This project aims at elucidating molecular mechanisms of cytokinesis and abscission. As model systems we use Drosophila melanogaster to understand how cytokinesis is regulated in a multi-cellular context in vivo, as well as human cultured cells. The project will apply a combination of cell biological, biochemical, molecular biological and genetic approaches as well as advanced microscopy.
The ESCRT (endosomal sorting complex required for transport)-III complex promotes membrane scission during cytokinetic abscission. We have recently elucidated that the scaffold protein ALIX interacts with ESCRT-III to mediate abscission in Drosophila female germline stem cells, showing an evolutionarily conserved role of the ALIX/ESCRT-III pathway in abscission. This project aims at further deciphering the molecular network within which ALIX acts during abscission by studying novel ALIX-interacting proteins. The project will address the molecular interactions, spatiotemporal control and functional roles of ALIX and candidate interacting proteins during cytokinesis in vivo and in human cultured cells.
The project will be performed in the project group of Kaisa Haglund (https://www.ous-research.no/haglund/) located within the group of Prof. Harald Stenmark at the Department of Molecular Cell Biology (https://www.ous-research.no/mcb/), Institute for Cancer Research and Centre for Cancer Cell Reprogramming (CanCell) (https://www.med.uio.no/cancell/english/). The Institute for Cancer Research plays a central role within the field of cancer research both in Norway and internationally. The Stenmark group has special expertise in microscopy of living and fixed cells and runs two of Norway’s most prominent facilities within advanced light and electron microscopy. The laboratory is located in the modern research building at the Institute for Cancer Research.