Methods. An in vivo study has been performed with DEE from a Euro 5-classified diesel engine running on three blends of diesel: petrodiesel fuel containing 7% (B7) or 20% (B20) 1st generation biodiesel made from rapeseed methyl ester, or 7% 1st generation and 13% 2nd generation biodiesel made from hydrogenated vegetable oil (HVO) (SHB20), both with and without diesel particulate filter (DPF). The animals were exposed to DEE for 1 day (6h/day), 7 days (6h/day), and for 28 days (6h/day, 5 days/week), both with and without particle filter. The animals (n=7/treatment) were exposed in whole body exposure chambers. In addition, a separate group exposed to NO2 is included to elucidate possible effects of this gas in the exhaust. Animals breathing clean air was used as controls. BAT and VAT has been isolated from the animals and we will analyse markers of inflammation and macrophage polarisation, mitochondrial function and production of adipokines regulating appetite and energy metabolism. In addition, we will use an adipocyte model where human mesenchymal stem cells are differentiated in culture into adipocytes. In this model, both the differentiating process and the functioning of adipocytes can be studied. Cell culture and multiplex analysis of proteins will be a main methodology in this project, but also gene expression may be included.
In 2016/2017 a student from HiOA has worked on a MSc project examining neurotoxic effects in animals from the same rat inhalation study and an extension of this work is described for a parallel master project proposal submitted to UiO entitled "Neurotoxicity of exhaust emissions from biodiesel fuels in rats”.
Oppgaven kan utføres i tidsrommet: høst 2018-2020
Veileder(e) / Prosjektansvarlig(e): Birgitte Lindeman (hovedveileder), Oddvar Myhre, Johan Øvrevik
Adresse, arbeidssted: Folkehelseinstituttet
E-post Birgitte.lindeman@fhi.no
Telefonnr: 21076224
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