This course is discontinued

MF9430 – MicroRNA and transcription factors as determinants of cell phenotypes and stem cell differentiation

Schedule, syllabus and examination date

Choose semester

Course content

The course describes microRNA and transcription factor (TF) interactions as a key regulatory system in the determination of cell phenotype through regulation of marker gene expression. It also describes how microRNA signatures of cell phenotypes are determinant for normal organ functioning and pathophysiology.

Learning outcome

Practical laboratory course and bioinformatics analyses. The objective is to provide practical information on how microRNA and transcription factors (TFs) interact in differentiating stem cells, how to use a microRNA species as disease markers. Furthermore, different databases (including Mir@nt@n)) will be used as part of the strategy to identify important microRNA-vs-TF networks. Lastly, the students will get some hands on experience related to the anlyses of microRNA and TF expression, as well as traditional staining techniques identifying osteoblasts, chondrocytes and adipocytes differentiated from mesenchymal stem cells or fat tissue derived stem cells.

Admission

Ph.D. candidates and students at the Medical Student Research Programme will get first priority to the course. The maximum number of participant is 30.

Prerequisites

Formal prerequisite knowledge

Cellbiology, Biochemistry, Physiology, Embryology etc.

Recommended previous knowledge

Generegulation, signaling pathways

Teaching

Part 1:
• Introduction to course: Differentiation of stem cells to osteoblasts, chondrocytes and adipocytes
• Transfection of siRNAs to suppress mRNAs of transcriptionfactors important for osteoblast, chondrocyte and adipocyte differentiation on plated stem cells. Incubation for 3 days.
Part 2:
• Cell staining techniques, light microscopy, assessment of percentage of marker-positive surfaces (mineralization, alkaline phosphatase, proteoglycans, fat droplets) using predifferentiated osteoblasts, chondrocytes and adipocytes from 14 day cultures
Part 3:
• Isolation of total RNA from 3 days differentiated osteoblasts, chondrocytes, and adipocytes. Q-PCR analyses of transcription factors (TFs) and selected microRNAs

Examination

Grading: Will be based on seminar performances, demonstration of practical skills, an essay with title/subject chosen by the participant, and the results obtained in the “multiple choice” session.

Grading: Pass/fail.

The examination papers will be scanned through the ephorus program.

Explanations and appeals

Evaluation

The course is subject to continuous evaluation. At regular intervals we also ask students to participate in a more comprehensive evaluation.

Facts about this course

Credits

5

Level

PhD

Teaching

 

It is currently not decided when this course will be offered again

Teaching language

Norwegian/English