KJM4420 – Analytical chemistry. Separation methods I

Schedule, syllabus and examination date

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Course content

Chromatographic Theory, Thin Layer Chromatography (TLC), Column Chromatography, Gas Chromatography (GC), High Performance Liquid Chromatography (HPLC), Supercritical Fluid Chromatography (SFC) and Electrophoresis. The basis for the different separation principles and techniques will be addressed. In the laboratory samples will be analyzed by the student.

Learning outcome

The student is expected to become familiar with the most important separation methods, their basis and potential use. The student is expected to gain independent laboratory skills in the experimental techniques, and knowledge of the principles behind and the construction of the instrumentation that is used.


Students who are admitted to study programmes at UiO must each semester register which courses and exams they wish to sign up for in Studentweb.

If you are not already enrolled as a student at UiO, please see our information about admission requirements and procedures.

Overlapping courses

10 credits overlap with KJM3420 – Analytical chemistry. Separation methods I (discontinued)


The course includes 20 hours of lectures, 8 hours of exercises and a mandatory laboratory course which consists of 7 exercises (80 laboratory hours). Approved laboratory course is a prerequisite for taking the exam.

The first lecture and laboratory lessons are mandatory. You therefore have to be registered at the first lecture and at the first laboratory lesson. If you are unable to join the mandatory lessons, you have to inform the Expedition Office in advance (phone 22 85 54 46 e-mail ekspedisjonen@kjemi.uio.no). If you fail to register in either of these ways, you will loose your access to the group lectures and to the examinations for the semester.


Written (4 hours) or oral examination. Letter grades (A-F).

This course offers new examination in the beginning of the subsequent semester for candidates who fail or withdraw during an ordinary examination.

Information about examination rules.


The teaching will be cancelled if less than 3 students have registered for the course by the closing date for course registration in January.

The laboratory course is valid in 4 semesters after it was taken. After that time, a new laboratory course is required.

Facts about this course






The course is taught for the last time spring 2011 and will from autumn 2011 be replaced by KJM3400.

Teaching language

Norwegian (English on request)

English if requested by English-speaking master students, otherwise Norwegian.