MBV9510 – Biomolecular NMR Spectroscopy

The course will give a practical introduction to NMR spectroscopy and how it is used to obtain structures of peptides and small proteins.

Structure information is essential for understanding the function of a protein. For small proteins and peptides that are difficult or impossible to crystallize NMR spectroscopy is the method available for 3D structure determination.

In NMR spectroscopy resonance frequency, intensity and correlation is measured. These spectroscopic qualities contain structural information. It is this structural information that is used in structure calculations that in the end give the NMR structures. The information is available in the NMR spectra, however, to be use full one need to obtain NMR spectra and determine where NMR signals come from. The later is in NMR spectroscopy called the assignment process. The course will have lectures in biomolecular NMR acquisition and assignment of 1D and 2D NMR spectra of water containing samples.

PhD candidates from the University of Oslo should apply for classes and register for examinations through Studentweb.

If a course has limited intake capacity, priority will be given to PhD candidates who follow an individual education plan where this particular course is included. Some national researchers’ schools may have specific rules for ranking applicants for courses with limited intake capacity.

PhD candidates who have been admitted to another higher education institution must apply for a position as a visiting student within a given deadline.

•  22 lecture hours and 
• 38 hours of lab-exercises. The course is thought intensively for 9 consecutive days every second year.  Selected articles should be studied before course start. Selected exercises should be finished after the course and brought to the final exam. Estimated time for this activity: one week and a half. All teaching sessions are obligatory and constitute the curriculum together with hand-outs and pre-defined articles.

• Final oral examination counting 100% after course ending. Participation and laboratory journals must  be approved befor the exam.