MBV9520 – Advanced biomolecular NMR

The course will give a practical introduction to NMR spectroscopy and how it is used to obtain structures of small and large proteins.

Structure information is essential for understanding the function of a protein. For proteins that are difficult or impossible to crystallize NMR spectroscopy is the method available for 3D structure determination.

In NMR spectroscopy resonance frequency, intensity and correlation is measured. These spectroscopic qualities contain structural information. It is this structural information that is used in structure calculations that in the end give the NMR structures. The resolution obtainable from unlabeled NMR samples is not good enough for proteins for the information to be obtained. To obtain NMR structures of proteins it is practice to label the sample with 15N and 13C, as a result there is a whole array of NMR experiments that can be used to obtain structure information. These experiments use direct J-couplings trough bond to connect spins on neighboring nuclei. Experiments on labeled samples can be used to determine the structure of large proteins.

PhD candidates from the University of Oslo should apply for classes and register for examinations through Studentweb.

If a course has limited intake capacity, priority will be given to PhD candidates who follow an individual education plan where this particular course is included. Some national researchers’ schools may have specific rules for ranking applicants for courses with limited intake capacity.

PhD candidates who have been admitted to another higher education institution must apply for a position as a visiting student within a given deadline.

•  Final oral examination counting 100% after course ending Participation and laboratory journals must  be approved before the exam.