BIOS4020 – Methods in Molecular Biology and Biochemistry II
Changes in the course due to coronavirus
Autumn 2020 the exams of most courses at the MN Faculty will be conducted as digital home exams or oral exams, using the normal grading scale. The semester page for your course will be updated with any changes in the form of examination.
The course focus on methods in biochemistry and molecular biology. The course gives an introduction to central biochemical and biophysical methods for the study of proteins. The different methods give information about the primary, secondary and tertiary structure of proteins.
After completing this course we expect your competence to include the following:
- Know the theory for protein expression, purification, crystallisation, protein crystallography, UV-vis spectroscopy, CD spectroscopy, protein NMR, proteomics and mass spectrometry for the study of proteins.
- Have knowledge about how you can use the methods described above for the study of proteins.
Master how these methods are used in practice.
Experience in presenting results both in written form and orally in a scientific way
Admission to the course
Students admitted at UiO must apply for courses in Studentweb. Students enrolled in other Master's Degree Programmes can, on application, be admitted to the course if this is cleared by their own study programme.
Nordic citizens and applicants residing in the Nordic countries may apply to take this course as a single course student.
If you are not already enrolled as a student at UiO, please see our information about admission requirements and procedures for international applicants.
Formal prerequisite knowledge
Recommended previous knowledge
Requires basic competence in biochemistry and molecular biology equivalent to a bachelor degree in Bioscience.
- 10 credits overlap with MBV4020 – Methods in molecular biology and biochemistry II (continued).
- 5 credits overlap with KJB220.
This course consists of
laboratory work with reports and a presentation
The laboratory work consists of both wet lab. and computer lab., and is divided into three modules:
First module is a project «From gene to structure and function» which includes protein expression, purification, crystallisation, structure solving and spectroscopic characterisation (50%).
Second module focus on CD and protein NMR spectroscopy (20%).
Third module focus on proteomics and mass spectrometry (30%).
The results from the laboratory work within the three modules are handed-in/presented as report in article format, ordinary reports and oral presentation, respectively.
The course is taught as an intensive course over 5 week, with 95% compulsory participation.
Attendance is mandatory for the first lecture, also for those on the waiting list. You will lose your seat on the course if documentation for absence is not provided to the student administration email@example.com prior to the first lecture
As the teaching involves laboratory and/or field work, you should consider taking out a separate travel and personal risk insurance. Read about your insurance cover as a student. A student who has completed compulsory instruction and coursework and has had these approved, is not entitled to repeat that instruction and coursework. A student who has been admitted to a course, but who has not completed compulsory instruction and coursework or had these approved, is entitled to repeat that instruction and coursework, depending on available capacity.
Final written exam, 4 hours, which counts 100 % towards the finale grade.
This course has mandatory assignments that must be approved before you can sit the finale exam.
The laboratory reports, the report in article format and oral presentation must be approved in order to be eligible for the final exam. It will also be counted as one of the three attempts to sit the exam for this course, if you sit the exam for one of the following course: MBV4020 – Methods in molecular biology and biochemistry II (continued).
Examination support material
Language of examination
The examination text is given in English.You may submit your response in Norwegian, Swedish, Danish or English.
Grades are awarded on a pass/fail scale. Read more about the grading system.
Resit an examination
This course offers both postponed and resit of examination. Read more: