BIOS4020 – Methods in Molecular Biology and Biochemistry II
Schedule, syllabus and examination date
The course focus on methods in biochemistry and molecular biology. The course gives an introduction to central biochemical and biophysical methods for the study of proteins. The different methods give information about the primary, secondary and tertiary structure of proteins.
After completing this course we expect your competence to include the following:
- Know the theory for protein expression, purification, crystallisation, protein crystallography, UV-vis spectroscopy, CD spectroscopy, protein NMR, proteomics and mass spectrometry for the study of proteins.
- Have knowledge about how you can use the methods described above for the study of proteins.
Master how these methods are used in practice.
Experience in presenting results both in written form and orally in a scientific way
Students who are admitted to study programmes at UiO must each semester register which courses and exams they wish to sign up for in Studentweb.
If you are not already enrolled as a student at UiO, please see our information about admission requirements and procedures.
Formal prerequisite knowledge
Recommended previous knowledge
Requires basic competence in biochemistry and molecular biology equivalent to a bachelor degree in Bioscience.
10 credits overlap with MBV4020 – Methods in molecular biology and biochemistry II (continued)
The information about overlaps is not complete. Contact the Department for more information if necessary.
This course consists of
- laboratory work with reports and a presentation
The laboratory work consists of both wet lab. and computer lab., and is divided into three modules:
- First module is a project «From gene to structure and function» which includes protein expression, purification, crystallisation, structure solving and spectroscopic characterisation (50%).
- Second module focus on CD and protein NMR spectroscopy (20%).
- Third module focus on proteomics and mass spectrometry (30%).
The results from the laboratory work within the three modules are handed-in/presented as report in article format, ordinary reports and oral presentation, respectively.
The course is taught as an intensive course over 5 week, with 95% compulsory participation.
Attendance is mandatory for the first lecture, also for those on the waiting list. You will lose your seat on the course if documentation for valid absence is not provided to the student administration firstname.lastname@example.org prior to the first lecture
As the teaching involves laboratory and/or field work, you should consider taking out a separate travel and personal risk insurance. Read about your insurance cover as a student.
- three hours written exam one week after the end of the course.
The laboratory reports, the report in article format and oral presentation must be approved in order to be eligible for the final exam.
Examination support material
Language of examination
The examination text is given in English.You may submit your response in Norwegian, Swedish, Danish or English.
Grades are awarded on a pass/fail scale. Read more about the grading system.
Explanations and appeals
Resit an examination
This course offers both postponed and resit of examination. Read more:
Withdrawal from an examination
It is possible to take the exam up to 3 times. If you withdraw from the exam after the deadline or during the exam, this will be counted as an examination attempt.
It will also be counted as one of the three attempts to sit the exam for this course, if you sit the exam for one of the following courses: MBV4020 – Methods in molecular biology and biochemistry II (continued)
Special examination arrangements
Application form, deadline and requirements for special examination arrangements.
The course is subject to continuous evaluation. At regular intervals we also ask students to participate in a more comprehensive evaluation.