KJM4350 – Protein crystallography
Schedule, syllabus and examination date
KJM4350 is a very challenging course, giving a thorough introduction to protein crystallography, the most powerful method to study protein structures and biomolecular complexes. The method will be studied in theory and practice, giving a solid foundation for a Masters or PhD project in the subject. The course shall also enable participants to read and judge modern literature in the field.
After completing the course, you shall have the following competence:
- Fundamental understanding of protein crystallography
- Know important terms and concepts like symmetry, space groups, crystals and crystallization, diffraction, Bragg's law, phase problem, Fourier synthesis and Fourier analysis, structure determination by multiple isomorphous replacement (MIR), multiple anomalous dispersion (MAD) and molecular replacement (MR), Patterson function, Patterson maps and electron density maps, refinement and validation.
- Gain practical experience related to protein structure determination by X-ray
- Get insight into complementary methods for macromolecular structure determination, such as electron diffraction and nuclear magnetic resonance (NMR) spectroscopy
- Be able to carry our relevant database searches
- Be able to read, understand and judge a crystallographic publication
Students who are admitted to study programmes at UiO must each semester register which courses and exams they wish to sign up for in Studentweb.
If you are not already enrolled as a student at UiO, please see our information about admission requirements and procedures.
Recommended previous knowledge
Basic knowledge of proteins and proficiency in mathematics is recommended.
The course comprises of 27 hours of lectures and 30 hours of compulsory theoretical exercises and discussion session, 8 hours of compulsory laboratory practicals, and a compulsory literature task that students are to present orally as well as in writing. The project task has to be approved before you can enter the final examination.
The first lecture is mandatory. If you are unable to join, you have to inform the Expedition Office in advance (phone 22 85 54 46). If not, you will loose the access to the course and to the examination.
Teaching takes place throughout the semester.
As the teaching involves laboratory and/or field work, you should consider taking out a separate travel and personal risk insurance. Read about your insurance cover as a student.
Access to teaching
A student who has completed compulsory instruction and coursework and has had these approved, is not entitled to repeat that instruction and coursework. A student who has been admitted to a course, but who has not completed compulsory instruction and coursework or had these approved, is entitled to repeat that instruction and coursework, depending on available capacity.
Presentation of a literature task, orally and in writing. Prepared participation in theoretical exercises and discussion session. Written summary of laboratory practicals (not to exceed 2 pages/report). Oral examination.
Examination support material
No examination support material is allowed.
Language of examination
You may submit your response in Norwegian, Swedish, Danish or English. If you would prefer to have the exam text in English, you may apply to the course administrators.
Grades are awarded on a scale from A to F, where A is the best grade and F is a fail. Read more about the grading system.
Explanations and appeals
Resit an examination
This course offers both postponed and resit of examination. Read more:
Withdrawal from an examination
It is possible to take the exam up to 3 times. If you withdraw from the exam after the deadline or during the exam, this will be counted as an examination attempt.
Special examination arrangements
Application form, deadline and requirements for special examination arrangements.
Written exercises are only valid for one semester.